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alpha tubulin recombinant antibody  (Proteintech)


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    Proteintech alpha tubulin recombinant antibody
    Alpha Tubulin Recombinant Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpha+tubulin+recombinant+antibody/pm41747744-34-64-101?v=Proteintech
    Average 96 stars, based on 268 article reviews
    alpha tubulin recombinant antibody - by Bioz Stars, 2026-08
    96/100 stars

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    Validation of gcKPNB3 silencing efficiency and subcellular/ total protein level analyses of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( A ) qRT-PCR analysis of gcKPNB3 silencing efficiency in CIK cells transfected with si-gcKPNB3-1, −2, or −3. ** P < 0.01. ( B ) Nuclear levels of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( C ) Cytoplasmic levels of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( D ) Total protein levels of endogenous IRF3 mediated by gcDDX56 and/or gcKPNB3 in GCRV-infected cells (MOI = 1) with overexpression and/or knockdown of gcDDX56 and/or gcKPNB3. <t>Tubulin</t> (cytoplasmic) and HDAC1 (nuclear) served as fractionation controls; GAPDH as internal control. Endogenous IRF3 was detected with anti-IRF3 antibodies. Band intensities were quantified via Image J.
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    Validation of gcKPNB3 silencing efficiency and subcellular/ total protein level analyses of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( A ) qRT-PCR analysis of gcKPNB3 silencing efficiency in CIK cells transfected with si-gcKPNB3-1, −2, or −3. ** P < 0.01. ( B ) Nuclear levels of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( C ) Cytoplasmic levels of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( D ) Total protein levels of endogenous IRF3 mediated by gcDDX56 and/or gcKPNB3 in GCRV-infected cells (MOI = 1) with overexpression and/or knockdown of gcDDX56 and/or gcKPNB3. <t>Tubulin</t> (cytoplasmic) and HDAC1 (nuclear) served as fractionation controls; GAPDH as internal control. Endogenous IRF3 was detected with anti-IRF3 antibodies. Band intensities were quantified via Image J.
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    Validation of gcKPNB3 silencing efficiency and subcellular/ total protein level analyses of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( A ) qRT-PCR analysis of gcKPNB3 silencing efficiency in CIK cells transfected with si-gcKPNB3-1, −2, or −3. ** P < 0.01. ( B ) Nuclear levels of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( C ) Cytoplasmic levels of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( D ) Total protein levels of endogenous IRF3 mediated by gcDDX56 and/or gcKPNB3 in GCRV-infected cells (MOI = 1) with overexpression and/or knockdown of gcDDX56 and/or gcKPNB3. Tubulin (cytoplasmic) and HDAC1 (nuclear) served as fractionation controls; GAPDH as internal control. Endogenous IRF3 was detected with anti-IRF3 antibodies. Band intensities were quantified via Image J.

    Journal: Journal of Virology

    Article Title: DEAD-box helicase gcDDX56 disrupts IRF3 nuclear import complex and promotes nuclear IRF3 degradation for enhancing GCRV replication

    doi: 10.1128/jvi.01654-25

    Figure Lengend Snippet: Validation of gcKPNB3 silencing efficiency and subcellular/ total protein level analyses of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( A ) qRT-PCR analysis of gcKPNB3 silencing efficiency in CIK cells transfected with si-gcKPNB3-1, −2, or −3. ** P < 0.01. ( B ) Nuclear levels of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( C ) Cytoplasmic levels of gcIRF3 mediated by gcDDX56 and/or gcKPNB3. ( D ) Total protein levels of endogenous IRF3 mediated by gcDDX56 and/or gcKPNB3 in GCRV-infected cells (MOI = 1) with overexpression and/or knockdown of gcDDX56 and/or gcKPNB3. Tubulin (cytoplasmic) and HDAC1 (nuclear) served as fractionation controls; GAPDH as internal control. Endogenous IRF3 was detected with anti-IRF3 antibodies. Band intensities were quantified via Image J.

    Article Snippet: The anti-HA rabbit polyclonal antibody (polyAb) (#51064-2-AP), anti-GAPDH mouse monoclonal antibody (mcAb) (#60004-1-Ig), and anti-Alpha Tubulin recombinant antibody (RecAb) (#80762-1-RR) were obtained from Proteintech.

    Techniques: Biomarker Discovery, Quantitative RT-PCR, Transfection, Infection, Over Expression, Knockdown, Fractionation, Control